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Dialysis bags Instructions
1.Product introduction
High-precision, ready-to-use dialysis bags that require no treatment prior to use (biological grade, sulfide and heavy metal ions removed, stored in embalming fluid)
There is no heavy metal pollution and sulfides in the manufacturing process of ready-to - use dialysis bags, which can be used without pretreatment and only need to be cleaned with deionized water to meet the application of accurate interception of molecular weight and membrane purity.
Advantages:
(1). Ductile film material, not easy to damage
(2). The impurity content is very small, can be used without pretreatment, only with deionized water cleaning
(3). Uniform aperture standard
(4). Small adsorption of solute
(5). Wide range of optional molecular weight 100-300000
(6). A variety of flat width options
(7). Unique biotechnology membrane, free of sulfide and metal impurities
2.Ordinary dry dialysis bag (United carbide, glycerine coating, need to be treated before use)
Technical parameters:
(1). PH stability range: 5-9
(2). Pollutant levels: sulphide & LT; 0.3%; Heavy metal & lt; 50ppm
(3). Chemical compatibility: Compatible with many salts, such as CaCl2, (NH4)2SO4; It is also compatible with water and organic solvents commonly used in molecular biology and enzymology, such as isopropyl alcohol, ethanol and acetone.
(4). Temperature resistance: it can be boiled and autoclave.
(5). Protein adsorption: the amount of adsorbed protein per gram of dialysis bag is less than 1ng.
Treatment of dry dialysis bag before use:
(1). Cut the dialysis bag into small pieces of appropriate length (10-20cm).
(2). Boil the dialysis bag in a large volume of 2%(W/V) sodium bicarbonate and 1mmol/L EDTA(pH 8.0) for 10 minutes.
(3). Thoroughly clean the dialysis bag with distilled water.
(4). Boil it in 1mmol/L EDTA(pH 8.0) for 10 minutes.
(5). After cooling, store at 4 ° C and ensure that the dialysis bag is always immersed in the solution. From now on, gloves are required to access dialysis bags.
(6). Fill the dialysis bag with water and drain it before use, then clean it.
Experimental requirements are not high can be used in a simple treatment method: boil in boiling water for 10min can be used.
Application of dialysis bag:
(1). Remove salts, surfactants and solvents
(2). Protein concentrate, peptides and antibodies
(3). Preparation of diluted protein before electrophoresis
(4). Combine research
(5). Buffer replacement and PH adjustment of sample solution
(6). Electroelution DNA
(7). Removal of small molecular pollutants
(8). Extraction and purification of tissue culture
Dialysis bag selection
1, The correct selection of appropriate interception molecular weight
The selection of molecular weight of interception is based on the molecular weight of the macromolecules reserved in the membrane and the molecular weight of the small molecule contaminants to be removed. To achieve reasonable and effective separation, the molecular weight ratio of the two substances to be separated should be at least 25.
The rule of thumb for choosing the molecular weight of interception is to select a molecular weight of approximately half of the molecular weight of the macromolecule to be retained to obtain at least 90% retention.
2, The correct choice of flat width
The selection of flat width of dialysis bag depends on sample volume and dialysis capacity. Smaller tubes dialysis faster; The larger dialysis tube is slower because of the longer diffusion distance. For ease of use, it is recommended to use a dialysis bag with a total length (including closing clip and top space) of approximately 10-15 cm.
3, The chemical compatibility of dialysis bags
Chemical compatibility is mainly used as a guide, not as a guarantee of chemical compatibility. Changes in temperature, concentration, and long exposure may affect the use of the product. You are advised to test your own conditions.
Use of dialysis bags
The following dialysis procedure is a common basic dialysis procedure. There are many variables that should be considered before starting dialysis. Variables such as the sample solvent, the chemical compatibility of the membrane, the molecular weight of the membrane, the dialysis solvent, the dialysis volume, and the temperature all affect the dialysis rate. Therefore, the following dialysis procedures may require appropriate changes in some applications.
(1) .Add an appropriate amount of dialysate (buffer) to the dialysis unit. The dialysate volume should be 100 times the sample volume. (e.g., 10 ml of dialysate in 1 liter of dialysate)
(2).Cut out the dialysis tube of appropriate length. Reserve an additional length (approximately 20% of the total sample volume) for head space.
(3).Insert the dialysis tube into the open dialysis clip and clamp again about 3-5 mm beyond the dialysis clip. Do not fold the dialysis bag.
(4).The sample is loaded at the open end of the dialysis tube. Adjust the length of the top space and tighten the dialysis clip.
(5).Place the dialysis sample in a suitable dialysis buffer.
(6).Dialysis should be based on specific application needs. Usually, overnight dialysis is allowed. Complete fluid replacement should be performed at least three times during continuous dialysis. Dialysate replacement is recommended at 2-4 hours, 6-8 hours, and 10-14 hours (morning of the second day). Continue dialysis for at least 2 hours after the last dialysate change.
Note: For high concentrations of contaminants, samples may require longer dialysis time and dialysate fluid replacement more frequently.
(7).The dialysis temperature depends on the sample. The temperature limit depends largely on the type of membrane. Cellulose dialysis bags can withstand temperatures up to 37℃, and regenerative cellulose dialysis bags can withstand temperatures up to 60℃.
Storage and shelf life of dialysis bags:
The shelf life is two years under proper storage conditions. Dry dialysis bags should be stored in polyethylene bags at room temperature or 4℃. Unopened dialysis bag stored at 4℃. Once wet, the dialysis bag should be immersed in one of the following solutions: 0.05% sodium azide, 1% sodium benzoate or 1% formaldehyde.
(Note: Once moist, do not let the dialysis bag dry. Continuous drying will cause the collapse of the pore structure irretrievably.)
Dialysis bags Instructions
1.Product introduction
High-precision, ready-to-use dialysis bags that require no treatment prior to use (biological grade, sulfide and heavy metal ions removed, stored in embalming fluid)
There is no heavy metal pollution and sulfides in the manufacturing process of ready-to - use dialysis bags, which can be used without pretreatment and only need to be cleaned with deionized water to meet the application of accurate interception of molecular weight and membrane purity.
Advantages:
(1). Ductile film material, not easy to damage
(2). The impurity content is very small, can be used without pretreatment, only with deionized water cleaning
(3). Uniform aperture standard
(4). Small adsorption of solute
(5). Wide range of optional molecular weight 100-300000
(6). A variety of flat width options
(7). Unique biotechnology membrane, free of sulfide and metal impurities
2.Ordinary dry dialysis bag (United carbide, glycerine coating, need to be treated before use)
Technical parameters:
(1). PH stability range: 5-9
(2). Pollutant levels: sulphide & LT; 0.3%; Heavy metal & lt; 50ppm
(3). Chemical compatibility: Compatible with many salts, such as CaCl2, (NH4)2SO4; It is also compatible with water and organic solvents commonly used in molecular biology and enzymology, such as isopropyl alcohol, ethanol and acetone.
(4). Temperature resistance: it can be boiled and autoclave.
(5). Protein adsorption: the amount of adsorbed protein per gram of dialysis bag is less than 1ng.
Treatment of dry dialysis bag before use:
(1). Cut the dialysis bag into small pieces of appropriate length (10-20cm).
(2). Boil the dialysis bag in a large volume of 2%(W/V) sodium bicarbonate and 1mmol/L EDTA(pH 8.0) for 10 minutes.
(3). Thoroughly clean the dialysis bag with distilled water.
(4). Boil it in 1mmol/L EDTA(pH 8.0) for 10 minutes.
(5). After cooling, store at 4 ° C and ensure that the dialysis bag is always immersed in the solution. From now on, gloves are required to access dialysis bags.
(6). Fill the dialysis bag with water and drain it before use, then clean it.
Experimental requirements are not high can be used in a simple treatment method: boil in boiling water for 10min can be used.
Application of dialysis bag:
(1). Remove salts, surfactants and solvents
(2). Protein concentrate, peptides and antibodies
(3). Preparation of diluted protein before electrophoresis
(4). Combine research
(5). Buffer replacement and PH adjustment of sample solution
(6). Electroelution DNA
(7). Removal of small molecular pollutants
(8). Extraction and purification of tissue culture
Dialysis bag selection
1, The correct selection of appropriate interception molecular weight
The selection of molecular weight of interception is based on the molecular weight of the macromolecules reserved in the membrane and the molecular weight of the small molecule contaminants to be removed. To achieve reasonable and effective separation, the molecular weight ratio of the two substances to be separated should be at least 25.
The rule of thumb for choosing the molecular weight of interception is to select a molecular weight of approximately half of the molecular weight of the macromolecule to be retained to obtain at least 90% retention.
2, The correct choice of flat width
The selection of flat width of dialysis bag depends on sample volume and dialysis capacity. Smaller tubes dialysis faster; The larger dialysis tube is slower because of the longer diffusion distance. For ease of use, it is recommended to use a dialysis bag with a total length (including closing clip and top space) of approximately 10-15 cm.
3, The chemical compatibility of dialysis bags
Chemical compatibility is mainly used as a guide, not as a guarantee of chemical compatibility. Changes in temperature, concentration, and long exposure may affect the use of the product. You are advised to test your own conditions.
Use of dialysis bags
The following dialysis procedure is a common basic dialysis procedure. There are many variables that should be considered before starting dialysis. Variables such as the sample solvent, the chemical compatibility of the membrane, the molecular weight of the membrane, the dialysis solvent, the dialysis volume, and the temperature all affect the dialysis rate. Therefore, the following dialysis procedures may require appropriate changes in some applications.
(1) .Add an appropriate amount of dialysate (buffer) to the dialysis unit. The dialysate volume should be 100 times the sample volume. (e.g., 10 ml of dialysate in 1 liter of dialysate)
(2).Cut out the dialysis tube of appropriate length. Reserve an additional length (approximately 20% of the total sample volume) for head space.
(3).Insert the dialysis tube into the open dialysis clip and clamp again about 3-5 mm beyond the dialysis clip. Do not fold the dialysis bag.
(4).The sample is loaded at the open end of the dialysis tube. Adjust the length of the top space and tighten the dialysis clip.
(5).Place the dialysis sample in a suitable dialysis buffer.
(6).Dialysis should be based on specific application needs. Usually, overnight dialysis is allowed. Complete fluid replacement should be performed at least three times during continuous dialysis. Dialysate replacement is recommended at 2-4 hours, 6-8 hours, and 10-14 hours (morning of the second day). Continue dialysis for at least 2 hours after the last dialysate change.
Note: For high concentrations of contaminants, samples may require longer dialysis time and dialysate fluid replacement more frequently.
(7).The dialysis temperature depends on the sample. The temperature limit depends largely on the type of membrane. Cellulose dialysis bags can withstand temperatures up to 37℃, and regenerative cellulose dialysis bags can withstand temperatures up to 60℃.
Storage and shelf life of dialysis bags:
The shelf life is two years under proper storage conditions. Dry dialysis bags should be stored in polyethylene bags at room temperature or 4℃. Unopened dialysis bag stored at 4℃. Once wet, the dialysis bag should be immersed in one of the following solutions: 0.05% sodium azide, 1% sodium benzoate or 1% formaldehyde.
(Note: Once moist, do not let the dialysis bag dry. Continuous drying will cause the collapse of the pore structure irretrievably.)